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Current Category » Introduction of Plant Biotechnology

Induction of Embryogenic Cell Suspension

The callus obtained from cultured explants is sliced and teased apart into small pieces which are then incubated in a liquid medium. Once a good embryogenic suspension has been established, somatic embryos can be obtained either by allowing the culture to age or by incubating the embryogenic tissue in a medium without 2,4-D. Since an unspplemented basal medium often encourages root formation, the normal practise is to add 500ml glutamine with 0.1 µm ABA to the medium to facilitate the embryo development.

Current Category » Introduction of Plant Biotechnology