Advantages and Limitations of Micropropagation
Advantages of Micropropagation:
1. Requires relatively small growing space.
2. The technique of micropropagation is applied with the objective of enhancing the rate of multiplication. Through tissue culture over a million plants can be grown from a small, even microscopic, piece of plant tissue within 12 months.
3. Shoot multiplication usually has a short cycle (2-6 weeks) and each cycle results in logarithmic increase in number of shoots.
4. Tissue culture gives propagules such as minitubers or microcorms for plant multiplication throughout the irrespective of the season.
5. The small size of propagules and their ability to proliferate in a soil free environment facilitate their storage on a large scale ability to proliferate in a soil free environment facilitate their storage on a large scale and also allows their large scale dissemination by suitable means of transport across international boundries
6. Stocks of germplasm can be main for many years.
7. Pathogen free plants can be raised and maintained economically.
8. Clonal propagation in dioecious is extremely important since seed progently yields 50% females and 50% male plants of one sex are desired commercially. For example, male plants of asparagus officinalis are more valuable then female plants. Propagation by stem cutting in male asparagus is not successful but can be achieved by tissue culture.
Limitations of Micropropagation:
1. Sophisticated facilities are required.
2. Demands greater skill in handling and maintenance than conventional techniques.
3. Shoot-tip derived plants may show genetic instability, E.g. 6-35% of banana clones developed through shoot tip culture show morphological variation.