Limitation of Somatic Hybridization
1. Intergeneric crosses between widely related plants which are not compatible sexually are not possible.
2. In certain wide crosses, elimination of chromosomes from hybrid cell is another limitation of somatic hybridisation.
3. In protoplast fusion experiments, the percentage of fusion between two different parental protoplast is very low.
4. For hybrid identification, selection and isolation at the culture level, there is no standardized method which is applicable for all material.