Plant Tissue Culture

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Plant Tissue Culture

The term “Plant tissue culture” broadly refers to the in vitro cultivation of plant parts under aseptic conditions. Such parts as meristems, apices, axillary buds. Young inflorescence, leaves, stems, and roots have been cultured. A controlled aseptic environment and suitable nutrient medium are the two chief requirements for successful tissue culture. These essential nutrients include inorganic salts, a carbon and energy source, vitamins and growth regulators.

The basic technology can be divided into five classes, depending on the material being used: Callus, organ, meristem, and protoplast and cell culture. The technique of embryo, ovule, ovary, anther and microspore culture are used and can yield genotypes that cannot easily be produced by conventional methodology.

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